Isolering och kultur av primära aortic endotelceller från miniatyr svin

Article DOI: 10.3791/59673-v • 06:38 min • August 14th, 2019

August 14th, 2019 •

Yanli Zhao^1,2,3, Chengjiang Zhao⁴, David K.C. Cooper⁵, Ying Lu², Kewang Luo⁶, Huiyun Wang³, Pengfei Chen³, Changchun Zeng³, Shaodong Luan¹, Lisha Mou², Hanchao Gao^1,2,3

¹Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ²Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, ³Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ⁴Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ⁵Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, ⁶People's Hospital of Longhua

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En effektiv enzymatisk metod för isolering av primära svin aorta endotelceller (pAECs) från miniatyr grisar beskrivs. De isolerade primära pAECs kan användas för att undersöka immun-och koagulations reaktionen vid xenotransplantation.

Isolering och kultur av primära aortic endotelceller från miniatyr svin

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