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A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
 

A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice

Article DOI: 10.3791/60095-v • 08:44 min • April 19th, 2020
April 19th, 2020 •
1, 2, 1,3, 1,3
1Centro de Investigación Príncipe Felipe (CIPF), 2Department of Physiology, Development and Neuroscience, University of Cambridge, 3Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM)

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We present a robust, cost-effective, and flexible method for measuring changes in hepatocyte number and nuclear ploidy within fixed/cryopreserved tissue samples that does not require flow cytometry. Our approach provides a powerful sample-wide signature of liver cytology ideal for tracking the progression of liver injury and disease.

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High-throughput In Situ Method Estimation Hepatocyte Nuclear Ploidy Mice Liver Development Regeneration Chronic Disease Automated 2D Tissue Sections Nuclear DNA Content Circular Hepatocyte Nuclei Aging Chronic Liver Disease Cancer Fixed Specimens Cryopreserved Specimens Research Tool Clinical Tool Liver Disease Progression Tissue Desegregation Fresh Material Harvesting Optimal Cutting Temperature Medium Cryomold Rapid Freezing Frozen Liver Lobule Sample Micrometer Thickness Polyamide Coated Slide Immunolabeling
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