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Journal / Developmental Biology / 神经冠状原脂肪衍生干细胞的分离、培养和异位诱导，来自围阴脂肪组织…
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Developmental Biology

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JoVE Journal Developmental Biology
神经冠状原脂肪衍生干细胞的分离、培养和异位诱导，来自围阴脂肪组织
 
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神经冠状原脂肪衍生干细胞的分离、培养和异位诱导，来自围阴脂肪组织

Article DOI: 10.3791/60691-v • 08:31 min • March 2nd, 2020
March 2nd, 2020 •
*1, *2, 3, 3, 4, 5, 4, 5, 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
* These authors contributed equally

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我们提出了从Wnt-1 Cre+/-的围阴脂肪组织中提取神经峰衍生的干细胞（NCADSCs）的分离、培养和异位诱导方案;罗莎26RFP/+小鼠。NCADSCs 是 ADSC 的一个容易获取的来源，用于在体外模拟脂肪发生或脂肪发生。

Tags

发育生物学， 问题 157， 神经峰细胞， Wnt-1， 小鼠， 围阴囊脂肪组织， 脂肪衍生基质细胞， 基质血管分数， 细胞培养， 阴囊诱导
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