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JoVE Journal Developmental Biology
High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function
 

High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function

Article DOI: 10.3791/60976-v • 08:34 min • March 12th, 2020
March 12th, 2020 •
1, 1, 2, 1, 2, 1, 1, 1
1Stanford Cardiovascular Institute, Stanford University, 2Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School

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We describe a protocol to assess heart morphology and function in adult zebrafish using high-frequency echocardiography. The method allows visualization of the heart and subsequent quantification of functional parameters, such as heart rate (HR), cardiac output (CO), fractional area change (FAC), ejection fraction (EF), and blood inflow and outflow velocities.

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High-frequency Ultrasound Echocardiography Zebrafish Cardiac Function Noninvasive High-resolution Representation Adult Heart Disease Models Potential Drug Screens Rapid Positioning Accurate Results Underwater Imaging Image Acquisition Platform Setup Glass Container Transducer Insertion Micromanipulator Holder Tricaine Methanesulfonate Transducer Rail System Control Software
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