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在跨脊柱直流刺激期间类型识别大鼠脊柱腺瘤的 Vivo 细胞内记录
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JoVE Revista Neurociencias
In Vivo Intracellular Recording of Type-Identified Rat Spinal Motoneurons During Trans-Spinal Direct Current Stimulation
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在跨脊柱直流刺激期间类型识别大鼠脊柱腺瘤的 Vivo 细胞内记录

DOI:
10.3791/61439-v

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11:07 min

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May 11, 2020

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,
1Department of Neurobiology,Poznań University of Physical Education

Capítulos

  • 00:04Introduction
  • 00:45Intravenous Line Placement
  • 01:47Tracheal Tube Introduction
  • 02:24Hind Limb Nerve Dissection
  • 03:19Laminectomy
  • 03:50Recording and Stimulation Preparation
  • 04:46Surface Electrode Placement
  • 05:54Dura and Pia Mater Opening
  • 06:28Trans-Spinal Direct Current Stimulation (tsDCS) Electrode Placement and Motoneuron racking and Penetration
  • 07:07Motoneuron Membrane and Firing Property Recording and tsDCS
  • 08:37Results: Representative Action Potentials and Membrane Property Analyses
  • 10:24Conclusion

Summary

Traducción Automática

Traducción Automática

该协议描述了大鼠腰椎瘤的体内细胞内记录，同时具有跨脊柱直电流刺激。该方法使我们能够测量膜特性，并记录脊髓的腺体或阴极化之前、期间和之后有节奏的分子发射。

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Here Are The Key Keywords From The Text:Intracellular RecordingSpinal MotoneuronsTrans-spinal Direct Current StimulationIn VivoRatDissectionJugular VeinTracheal TubeHind Limb NervesSciatic NerveTibial NerveGastrocnemius Nerve

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