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JoVE Journal Bioengineering
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
 

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Article DOI: 10.3791/62599-v • 12:18 min • August 3rd, 2021
August 3rd, 2021 •
1, 2, 2
1Department of Chemical Engineering, Worcester Polytechnic Institute, 2Center for Biomedical Engineering, School of Engineering, Brown University

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This protocol describes the formation of cell mimicking uni-lipid and multi-lipid vesicles, supported lipid bilayers, and suspended lipid bilayers. These in vitro models can be adapted to incorporate a variety of lipid types and can be used to investigate various molecule and macromolecule interactions.

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Lipid Bilayer Molecular Interactions Pharmaceutical Agents Environmental Toxicants Biological Compounds Membrane Construction Compound Permeability Absorption Embedment Lipid Stock Solution Chloroform Removal Tris Sodium Chloride Buffer Vesicle Concentration Freezing And Thawing Mini Extruder
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