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Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts
 

Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts

Article DOI: 10.3791/62605-v • 07:16 min • May 14th, 2021
May 14th, 2021 •
1, 1, 1
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences

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This protocol for immunofluorescent labeling of both plant virus proteins and vector insect proteins in excised insect guts can be used to study interactions among virus and vector insects, insect protein functions and molecular mechanisms underlying virus transmission.

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Immunofluorescent Labeling Plant Virus Insect Vector Proteins Hemipteran Guts Movement In Insects Functions Of Insect Proteins Interactions Between Virus And Vector Insect Laser Scanning Confocal Microscopy Lu Zhang PhD Non-viruliferous Insects Southern Rice Black Streaked Dwarf Virus (SRBSDV) Virus Acquisition Access Period Rice Seedlings Dissection Excision Of The Gut 0.01 Molar PBS 4% Paraformaldehyde 2% Triton X-100 Glass Slide Optical Microscope SRBSDV Infected Adults 1.5 Milliliter Tube On Ice Paralyze The Insects
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