Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / From Cells to Cell-Free Protein Synthesis within…
JoVE Journal
Bioengineering

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Bioengineering
From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow
 

From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow

Article DOI: 10.3791/62866-v • 06:08 min • July 22nd, 2021
July 22nd, 2021 •
1,2, 1,2, 1,2, 1,2
1Department of Chemistry and Biochemistry, California Polytechnic State University San Luis Obispo, 2Center for Application in Biotechnology, California Polytechnic State University San Luis Obispo

Capítulos

Resumen
Automatic Translation

This work describes the preparation of cell extract from Escherichia coli (E. coli) followed by cell-free protein synthesis (CFPS) reactions in under 24 hours. Explanation of the cell-free autoinduction (CFAI) protocol details improvements made to reduce researcher oversight and increase quantities of cell extract obtained.

Tags

Cell-free Protein Synthesis Autoinduction Workflow In Vitro Protein Synthesis Cell-free Expression Energy Metabolism Cell-free Reactions Autoinduction Media Sugar Solution Inoculate Media E.coli BL21 Star Plate Incubator Centrifuge
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter