Journal
/
Neurociencias
/
6-羟基多巴胺诱导的基于斑马鱼的成人帕金森病模型的运动评估
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model
Please note that all translations are automatically generated. Click here for the English version.

6-羟基多巴胺诱导的基于斑马鱼的成人帕金森病模型的运动评估

DOI:
10.3791/63355-v

•

07:32 min

•

December 28, 2021

•

, , , , , ,
1Collaborative Drug Discovery Research (CDDR) Group and Brain Degeneration and Therapeutics Group, Faculty of Pharmacy,Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, 2Department of Medicine, Faculty of Medicine,Universiti Malaya

Capítulos

  • 00:05Introduction
  • 01:00Zebrafish Maintenance and Pre-ICV Microinjection Preparations
  • 02:19Anaesthetisation and ICV Injection of Zebrafish
  • 04:10Locomotor Assessment
  • 05:22Results: Assessment of Neuroregeneration in Zebrafish Based PD Model
  • 06:44Conclusion

Summary

Traducción Automática

Traducción Automática

本方案描述了在腹侧间脑（Dn）处用神经毒性6-羟基多巴胺（6-OHDA）向成年斑马鱼注射脑室（ICV）以及通过使用开放水箱测试评估损伤和随后恢复的游泳行为，并伴有使用视频跟踪软件进行分析。

Tags

ZebrafishParkinson’s Disease6-hydroxydopamineLocomotor AssessmentMotor ImpairmentDopaminergic NeuronsIntracerebroventricular InjectionNeuroregenerationCell ReplacementTricaine MethanesulfonateAnesthesiaMicrocapillary Injector

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

方法来描述自发和惊吓引起的步态中的鱼藤酮诱导的帕金森病模型<em>果蝇</em

Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease

胸脊髓截断手术和大鼠开放场运动评估

成人脑切片长期抑郁症诱导评估

将RatWalker系统应用于帕金森病遗传大鼠模型中的步态分析

帕金森病的6-羟基多巴胺大鼠模型

左旋多巴诱导的帕金森病大鼠模型中运动障碍的诱导与评估

成年斑马鱼的游泳耐力和游泳行为评估

分析编码人类α突触核蛋白的腺相关病毒载体诱导的帕金森病小鼠模型

使用MouseWalker量化脊髓损伤小鼠模型中的运动功能障碍

Read Article