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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

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A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

JoVE Revista
Biología

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JoVE Revista Biología

A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

DOI:

10.3791/65611-v

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01:58 min

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July 28, 2023

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Lucas Stalder, Fabian Koechl, Kerstin Hahn, Marc Sultan, Megana K. Prasad

¹Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

Tags

Single Nuclei Isolation Single Nucleus Sequencing Automated Nuclei Extraction Standardized Protocols Drug Discovery Transcriptomics Gene Expression Robotic Dissociation Chemical Gradient Filtration Automated Cell Counting Mouse Brain Rat Kidney Cynomolgus Liver And Spleen

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