Infection of Cotyledons with Agrobacterium rhizogenes Strain NCPPB2659 (K599), Selection, and Harvesting of Hairy Roots.

Infection of Cotyledons with Agrobacterium rhizogenes Strain NCPPB2659 (K599), Selection, and Harvesting of Hairy Roots. (Video) | JoVE

Published: May 05, 2023

doi:

10.3791/200165

Jie Lin, Dasol Wi, Melissa Ly, Md Asraful Jahan, Sarah Pullano, Izabella Martirosyan, Nik Kovinich

¹Department of Biology,York University, ²Department of Genetic Engineering and Biotechnology,Shahjalal University of Science and Technology

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Keywords: Agrobacterium Rhizogenes Soybean Cotyledons Hairy Roots TI Plasmid VirD2 PCR Spectinomycin Phosphate Buffer Acetosyringone Germination Medium Hairy Root Growth Elicitor Treatment Chemical Treatment RNA Extraction

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Infection of Cotyledons with Agrobacterium rhizogenes Strain NCPPB2659 (K599), Selection, and Harvesting of Hairy Roots.. J. Vis. Exp. (Pending Publication), e200165, doi:10.3791/200165 (2023).

Infection of Cotyledons with Agrobacterium rhizogenes Strain NCPPB2659 (K599), Selection, and Harvesting of Hairy Roots. (Video) | JoVE

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