Infection of Cotyledons with Agrobacterium rhizogenes Strain NCPPB2659 (K599), Selection, and Harvesting of Hairy Roots.

Infecção de Cotilédones com Agrobacterium rhizogenes Strain NCPPB2659 (K599), Seleção e Colheita de Raízes Peludas.

Published: May 05, 2023

doi:

10.3791/200165

Jie Lin, Dasol Wi, Melissa Ly, Md Asraful Jahan, Sarah Pullano, Izabella Martirosyan, Nik Kovinich

¹Department of Biology,York University, ²Department of Genetic Engineering and Biotechnology,Shahjalal University of Science and Technology

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Keywords: Agrobacterium Rhizogenes Soybean Cotyledons Hairy Roots TI Plasmid VirD2 PCR Spectinomycin Phosphate Buffer Acetosyringone Germination Medium Hairy Root Growth Elicitor Treatment Chemical Treatment RNA Extraction

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Infection of Cotyledons with Agrobacterium rhizogenes Strain NCPPB2659 (K599), Selection, and Harvesting of Hairy Roots.. J. Vis. Exp. (Pending Publication), e200165, doi:10.3791/200165 (2023).

Infecção de Cotilédones com Agrobacterium rhizogenes Strain NCPPB2659 (K599), Seleção e Colheita de Raízes Peludas.

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