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A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages

August 04, 2023
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High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes

00:50 min
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Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages

04:03 min
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1Department of Internal Medicine,University of California, Davis, 2Department of Medical Microbiology and Immunology,University of California, Davis

Summary

Traduction automatique

This protocol describes the procedure for genome editing in mouse bone marrow-derived macrophages using Cas9-sgRNA ribonucleoprotein complexes assembled in vitro and delivered by electroporation.

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