Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

June 23rd, 2023

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Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen^3,4, Kaelyn Sumigray^1,5,6

¹Department of Genetics, Yale School of Medicine, ²Department of Pediatrics, Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology, Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology, Yale School of Medicine, ⁵Yale Stem Cell Center, Yale School of Medicine, ⁶Yale Cancer Center, Yale School of Medicine

* These authors contributed equally

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Burada, sıçan bağırsak organoidleri üretmek ve bunları birkaç aşağı akış uygulamasında kullanmak için bir protokol sunuyoruz. Sıçanlar genellikle tercih edilen bir klinik öncesi modeldir ve sağlam bağırsak organoid sistemi, in vivo çalışmalara eşlik edecek bir in vitro sistem ihtiyacını karşılar.

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Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

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