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Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

June 23rd, 2023
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, 4Medical Research Center, Fujian Maternity and Child Health Hospital

Summary
Automatic Translation

Denne artikkelen rapporterer konstruksjonen av sentromerassosiert protein-E (CENP-E) knockoutceller ved hjelp av CRISPR/Cas9-systemet og tre fenotypebaserte screeningstrategier. Vi har benyttet CENP-E knockout-cellelinjen for å etablere en ny tilnærming for å validere spesifisiteten og toksisiteten til CENP-E-hemmerne, noe som er nyttig for stoffutvikling og biologisk forskning.

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