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Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei

January 19th, 2024
*1, *2,3, 1, 2,3, 2,4, 1, 2
1Department Chemistry and Biochemistry, Brigham Young University, 2Eukaryotic Pathogens Innovation Center, Clemson University, 3Department of Genetics and Biochemistry, Clemson University, 4Department of Physics and Astronomy, Clemson University
* These authors contributed equally

Summary
Automatic Translation

我们描述了一种研究pH值如何响应非洲锥虫血液形式糖体中环境线索的方法。该方法涉及将pH敏感的可遗传蛋白传感器与流式细胞术相结合，以测量pH动态，既可以作为时程测定，也可以作为高通量筛选形式。

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