Jiangnan University 5 articles published in JoVE Immunology and Infection Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo Xiaorun Zhai*1, Jiao Li*1, Yunjuan Nie1 1Department of Basic Medicine, Wuxi School of Medicine, Jiangnan University This protocol describes the isolation of pulmonary interstitial macrophages (IMs) and their adoptive transfer after IL-33 stimulation of the lung alveoli in a mouse model, which can facilitate the in vivo study of idiopathic pulmonary fibrosis (IPF). Chemistry Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions Philippe Icyishaka*1, Chenxing Li*1, Liushen Lu1, Wenhao Bao1, Jia Li1, Yan Zhang2, Yijian Rao1 1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 2School of Pharmaceutical Science, Jiangnan University New routes for the synthesis of nitrogen-containing heterocycles utilizing cercosporin as a metal-free photocatalyst were developed. Engineering O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression Zihao Chen1, Niannian Zheng1, Xiaoli Luan1, Fei Liu1 1Key Laboratory for Advanced Process Control of Light Industry of the Ministry of Education, Institute of Automation, Jiangnan University The protocol describes a method of predicting o-cresol concentration during the production of polyphenylene ether using near-infrared spectroscopy and partial least squares regression. To describe the process more clearly and completely, an example of predicting the o-cresol concentration during the production of polyphenylene is used to clarify the steps. Biochemistry Preparation of High-Quality Fermented Fish Product Fang Yang*1, Lu-Lu Zhu*1, Yu-Duan Diao1, Pei Gao1, Da-Wei Yu1, Pei-Pei Yu1, Qi-Xing Jiang1, Yan-Shun Xu1, Wen-Shui Xia1, Xiao-Bei Zhan2 1State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, 2The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University The goal of the protocol is to provide an industrialized fish fermentation technique based on inoculation of Saccharomyces cerevisiae. Bioengineering Ligand Nano-cluster Arrays in a Supported Lipid Bilayer Emmanuelle Benard1, Fuwei Pi1,2, Igor Ozerov1, Anne Charrier1, Kheya Sengupta1 1Aix-Marseille Université, CNRS, UMR 7325, CINaM, 2State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University We present a protocol to functionalize glass with nanometric protein patches surrounded by a fluid lipid bilayer. These substrates are compatible with advanced optical microscopy and are expected to serve as platform for cell adhesion and migration studies.