Tibet University 1 article published in JoVE Medicine A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging Yang Li*1,2, Xue Qiao*1, Xuechuan Hong1,2 1College of Science, Research Center for Ecology, Laboratory of Extreme Environmental Biological Resources and Adaptive Evolution, Tibet University, 2State Key Laboratory of Virology, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (MOE) and Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, Wuhan University School of Pharmaceutical Sciences The present protocol describes a detailed, real-time NIR-II fluorescence imaging operation of a mouse using a NIR-II optics imaging device.