Nanjing Forest Police College 1 article published in JoVE Biochemistry Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS Changhua Zhu*1, Xiaoye Liu*2, Wenlei Wang1, Xiaoguang Chen3, Shangyu Gao1, Meng Qian1, Na Yang1, Yifeng Xu1, Mingjia Chen1 1College of Life Sciences, Nanjing Agricultural University, 2Department of Criminal Science and Technology, Nanjing Forest Police College, 3Department of Molecular Nutrition and Biochemistry of Plants, Institute of Plant Nutrition, Leibniz University Hannover A precise and reproducible method for in vivo nucleosides/nucleotides quantification in plants is described here. This method employs an HPLC-MS/MS.