Northwest Normal University 1 article published in JoVE Developmental Biology Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish Wenyi Wang1, Tao Kang1, Lin Bai1, Wei Hu2, Yayoi Obata3, Jianzhen Li1 1College of Life Sciences, Northwest Normal University, 2State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 3Department of Bioscience, Tokyo University of Agriculture Here, we present a simple method for separating follicular cells and oocytes in zebrafish ovarian follicles, which will facilitate investigations of ovarian development in zebrafish.