Chengdu University of Traditional Chinese Medicine 21 articles published in JoVE Medicine Field Collection and Laboratory Routine Identification of Rhodiola crenulata Jiayi Wang*1, Na Xie*2,3, Mengjia Li1, Jinsong Su4, Ya Hou3, Yi Zhang1,2,3, Xiaobo Wang2 1School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, 2Meishan Hospital of Chengdu University of Traditional Chinese Medicine, 3School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 4Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine Here, we describe the identification of Rhodiola Crenulata from habitat, plant morphology, medicinal properties, microscopic features, and thin-layer chromatography. Neuroscience Barrier Functional Integrity Recording on bEnd.3 Vascular Endothelial Cells via Transendothelial Electrical Resistance Detection Fuhan Fan1, Hong Jiang1, Ya Hou1, Yi Zhang1,2,3, Qipeng Zhao4, Yong Zeng1,2, Xianli Meng1,2,5, Xiaobo Wang2 1School of Pharmacy/School of Modern Chinese Medicine Industry, Chengdu University of Traditional Chinese Medicine, 2Meishan Hospital of Chengdu University of Traditional Chinese Medicine, 3School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, 4School of Pharmacy, Ningxia Medical University, 5Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline, Chengdu University of Traditional Chinese Medicine This protocol describes a dependable and efficient in vitro model of the brain blood barrier. The method uses mouse cerebral vascular endothelial cells bEnd.3 and measures transmembrane electrical resistance. Medicine Fast Inspection of Quality of Indigo Naturalis by Multiple Light Scattering Xuemei Liu1, Hui Ye1, Haozhou Huang1, Ming Yang2, Li Han1, Runchun Xu1, Dingkun Zhang1 1School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2State Key Laboratory of Innovation Drug and Efficient Energy-saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine Here, we present a quality evaluation method based on a multiple light scattering technique to evaluate the quality of Indigo Naturalis. Medicine Acupoint Needle-Embedding Combined with Ironing Therapy for Postoperative Pain After Anal Surgery Xiao Tang1,2, Jian Kang1, Ya Liu1 1Hospital of Chengdu University of Traditional Chinese Medicine, 2Chengdu University of Traditional Chinese Medicine The high incidence of pain after anal surgery is due to the physiological structure and pathological status of the anus. At present, the use of painkillers cannot provide satisfactory pain relief, which makes complementary therapy important. This paper proposes acupoint needle-embedding combined with ironing therapy to relieve postoperative pain. Medicine Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method Liqiong Yu1, Shiling Li1, Xiaoyan Tan1, Chengping Wang2, Xianrong Lai1,2, Yue Liu2, Yi Zhang2 1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2State Key Laboratory of Southwestern Chinese Medicine Resources, School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine The present protocol describes an efficient method for optimization of the processing technology of Tiebangchui processed with highland barley wine based on a Box-Behnken design response surface combined with the entropy method. Medicine Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response Surface Method Shiling Li1, Liqiong Yu1, Congying Li2, Naiyu Wang1, Xianrong Lai2, Yue Liu2, Yi Zhang2 1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine The present protocol describes an efficient and standard detoxification processing method for Zanba-stir-fried Tiebangchui using CRITIC combined with the Box-Behnken response surface method. Medicine Objectification of Tongue Diagnosis in Traditional Medicine, Data Analysis, and Study Application Li Feng*1, Wenke Xiao*1, Chuanbiao Wen1, Qiaoling Deng1, Jinhong Guo1, Haibei Song1 1School of Intelligent Medicine, Chengdu University of Traditional Chinese Medicine The present study employed U-Net and other deep learning algorithms to segment a tongue image and compared the segmentation results to investigate the objectification of tongue diagnosis. Medicine Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods Haozhou Huang*1,2, Mengqi Li*3, Chuanhong Luo4, Sanhu Fan5, Taigang Mo5, Li Han4, Dingkun Zhang4, Junzhi Lin6 1State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline, Chengdu University of Traditional Chinese Medicine, 2Meishan Hospital of Chengdu University of Traditional Chinese Medicine, 3Sichuan Nursing Vocational College, 4State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 5Sanajon Pharmaceutical Group, 6TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine Here, we introduce a stability evaluation method based on multiple light scattering technology to evaluate the stability of traditional Chinese medicine extracts. Medicine Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia Baihan Zeng*1,2,3, Luming Qi*1,2,3, Sha Wu*1,2,3, Nannan Liu1,2,3, Jie Wang1,2,3, Kaidi Nie1,2,3, Lina Xia1,2,3, Shuguang Yu2,3 1School of Health Preservation and Rehabilitation, Chengdu University of Traditional Chinese Medicine, 2State Administration of Traditional Chinese Medicine Key Laboratory of Traditional Chinese Medicine, Regimen and Health, Chengdu University of Traditional Chinese Medicine, 3Key Laboratory of Traditional Chinese Medicine Regimen and Health of Sichuan Province The present protocol describes an integrated strategy for exploring the key targets and mechanisms of Fructus Phyllanthi against hyperlipidemia based on network pharmacology prediction and metabolomics verification. Biology Noninvasive Intratracheal Lipopolysaccharide Instillation in Mice Peng Yu*1, Bo Lin*2, Jingyang Li1, Yanqing Luo1, Di Zhang1, Jiayi Sun1, Xianli Meng1, Yingfan Hu3, Li Xiang1 1State Key Laboratory of Southwestern Chinese Medicine Resources, College of Pharmacy, Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Department of Pharmacy, The Second Affiliated Hospital of Hainan Medical University, 3The School of Preclinical Medicine, Chengdu University Here, we propose a protocol for intratracheal lipopolysaccharide (LPS) delivery via noninvasive oropharyngeal endotracheal intubation. This method minimizes the trauma of the surgical procedure for the animal and accurately delivers LPS to the trachea and then to the lungs. Chemistry Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis Xing Fu1, Yuan Pan1, Yili Wang1, Zhaoqing Pei1, Binjie Xu1, Jing Zhang2, Jinsong Su1 1State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 2School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine Here, we describe a general protocol and design that could be applied to identify trace amounts and minor constituents in the complex natural product formulations (matrixes) in Tibetan medicine. Chemistry An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry Hui Ye1, Xuemei Liu1, JiaBao Liao2, Haozhou Huang3, Lin Huang1, Yang Bao1, Hongyan Ma1, Junzhi Lin4, Xiaoming Bao5, Dingkun Zhang1, Runchun Xu1 1State Key Laboratory of Southwestern Chinese Medicine Resources, Pharmacy School, Chengdu University of Traditional Chinese Medicine, 2China Resources Sanjiu Modern Chinese Medicine Pharmaceutical Co., Ltd, 3Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 4TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, 5Shimadzu (China) Co., Ltd Here, a headspace gas chromatography-tandem quadrupole mass spectrometry (HS-GC-MS/MS) method suitable for the determination of trimethylamine (TMA) in animal-derived medicines is described. The protocol includes sample pretreatment, headspace treatment, analysis conditions, methodological validation, and the determination of TMA in animal-derived medicines. Medicine A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes Wei Wang*1, Dingxi Bai*1, Chenxi Wu*1, Hang Li1, Xuemei Xie1, Wenting Ji1, Jing Gao1 1College of Nursing, Chengdu University of Traditional Chinese Medicine The streptozotocin-induced diabetic wound model in male SD rats is currently the most widely used model for studying wound healing in type I diabetes mellitus. This protocol describes the methods used to construct this model. It also presents and addresses potential challenges and examines the progression and angiogenic characteristics of diabetic wounds. Medicine Meta-Analysis of the Effectiveness and Safety of Shugan Jieyu Capsules for the Treatment of Insomnia Jing Wang*1, Peipei Du*2, Linda LD Zhong2, Yaxuan Ren1, Li Feng1, Shu Yang1, Haibei Song1 1School of Intelligent Medicine, Chengdu University of Traditional Chinese Medicine, 2School of Chinese Medicine, Hong Kong Baptist University The presented protocol describes an investigation strategy and systematic review to evaluate the clinical effectiveness and safety of Shugan Jieyu capsules for treating insomnia. Medicine Enema of Traditional Chinese Medicine for Patients with Severe Acute Pancreatitis Shi Wang1,2, Tong Zhou1,2, Kunlan Long1, Jun Chen1, Xiujuan Zhou1, Peiyang Gao1 1Department of Critical Care Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, 2Clinical Medicine Department, Chengdu University of Traditional Chinese Medicine An enema is a type of traditional Chinese medicine (TCM) therapy used to treat severe acute pancreatitis (SAP). The therapy involves instilling a TCM liquid into the rectum and colon through the anus to keep the liquid in the intestine, absorbing through the intestinal mucosa. This study aims to standardize the enema procedure for SAP patients. Medicine Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI Binjie Xu1,4, Lirun Chen3, Fengqi Lv3, Yuan Pan1, Xing Fu1, Zhaoqing Pei1,2 1Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Tianfu Chinese Medicine Innovation Harbour, Chengdu University of Traditional Chinese Medicine, 3School of Medical Technology, Chengdu University of Traditional Chinese Medicine, 4China Resources Sanjiu (Ya’an) Pharmaceutical Co., Ltd. In this study, a series of methods are presented to prepare DESI-MSI samples from plants, and a procedure of DESI assembly installation, MSI data acquisition, and processing is described in detail. This protocol can be applied in several conditions for acquiring spatial metabolome information in plants. Medicine Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique Hong Jiang*1, Yating Zhang*1, Ya Hou1, Lijun Li2, Sanyin Zhang3,4, Yi Zhang5, Xianli Meng1,3,4, Xiaobo Wang4 1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Chengdu Techman Instrument Co., Ltd., 3Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 4Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, 5School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine The present protocol describes an efficient method for the real-time and dynamic acquisition of voltage-gated potassium (Kv) channel currents in H9c2 cardiomyocytes using the whole-cell patch-clamp technique. Medicine An In Vitro Dissolution Determination of Multi-Index Components in Tibetan Medicine Rhodiola Granules Qinyun Du1, Qingxiu He2, Fengming Zhang3, Jiao Mi3, Yuying Li3, Shaohui Wang1, Yi Zhang1 1School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, 3Tibet Rhodiola Pharmaceutical Holding Co. Ltd Here, we test the dissolution of Rhodiola granules (RG) in vitro, draw dissolution curves of salidroside, gallic acid, and ethyl gallate in ultrapure water, and fit the curves to different mathematical models. This protocol provides information and guidance for in vivo bioequivalence and in vivo-in vitro correlation studies of RG. Neuroscience Real-Time Dynamic Collection of Hippocampal Extracellular Fluid from Conscious Rats Using a Microdialysis System Xiaobo Wang1,2, Na Xie3, Yi Zhang4, Xianli Meng1,2,3, Ya Hou3, Sanyin Zhang1,2 1Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, 3School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 4School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine The protocol here provides a detailed real-time dynamic sampling of extracellular fluid from the hippocampus of awake rats using a microdialysis system. Medicine Dynamic Continuous Blood Extraction from Rat Heart via Noninvasive Microdialysis Technique Ya Hou1, Jinrong Bai1,2, Yi Zhang3, Xianli Meng1,2,4, Sanyin Zhang4, Xiaobo Wang2 1School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, 3School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, 4Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine The present protocol describes a simple and efficient method for the real-time and dynamic collection of rat heart blood using the microdialysis technique. Medicine Standardized Rat Coronary Ring Preparation and Real-Time Recording of Dynamic Tension Changes Along Vessel Diameter Pengmei Guo*1, Wenqiao An*2, Ying Guo1, Zhihao Sun2, Xiaobo Wang1,3, Sanyin Zhang1,3 1Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 2School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, 3Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine The present protocol describes the wire myograph technique for measuring vascular reactivity of the rat coronary artery.
