Beijing Graphene Institute 1 article published in JoVE Engineering Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection Sakib Ishraq1, Jianbo Sun2, Yuxin Liu1 1Lane Department of Computer Science and Electrical Engineering, West Virginia University, 2Beijing Graphene Institute The present protocol demonstrates the development of electrolyte-gated graphene field-effect transistor (EGGFET) biosensor and its application in biomarker immunoglobulin G (IgG) detection.