Hubei University of Chinese Medicine 1 article published in JoVE Medicine Improved Suspension Fixation Using Rat Jacket in Rat Acupuncture Experiments Qing Liu*1, Cheng-Kai Xiong*1, Bi-Yong Liu1, Min You1, Yan-Jun Du1, Jian-Min Liu1 1College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine Here, we present an improved suspension fixation method using a rat jacket to effectively immobilize rats during acupuncture experiments. Furthermore, the structure and application of this fixation apparatus, as well as instructions and evaluation for its utilization in electroacupuncture therapy, is elucidated.