The Hebrew University of Jerusalem 7 articles published in JoVE Biology Imaging Flow Cytometry to Study Microbial Autoaggregation Ronit Suissa1, Uzi Hadad2, Michael Meijler1, Ilana Kolodkin-Gal3,4 1Department of Chemistry, Ben-Gurion University of the Negev, 2Ilse Kats Institute for Nanoscale Science & Technology, Ben-Gurion University of the Negev, 3Department of Plant Pathology and Microbiology, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 4The Scojen institute for synthetic biology, Reichman university This protocol describes a quantitative approach to measure microbial autoaggregation using imaging flow cytometry. Cancer Research Pancreatic Tissue Dissection to Isolate Viable Single Cells Oshri Yosefov-Levi1, Sharona Tornovsky1, Oren Parnas1 1The Lautenberg Center for Immunology and Cancer Research, The Institute for Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalem Pancreatic metaplastic cells are precursors of malignant cells that give rise to pancreatic tumors. However, isolating intact viable pancreatic cells is challenging. Here, we present an efficient method for pancreatic tissue dissociation. The cells can then be used for single-cell RNA sequencing (scRNA-seq) or for two- or three-dimensional co-culturing. Biology Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes Vijigisha Srivastava1, Einav Gross1 1Department of Biochemistry and Molecular Biology, Faculty of Medicine, Institute for Medical Research, Israel-Canada (IMRIC), The Hebrew University of Jerusalem Exploring mitophagy through electron microscopy, genetic sensors, and immunofluorescence requires costly equipment, skilled personnel, and a significant time investment. Here, we demonstrate the efficacy of a commercial fluorescence dye kit in quantifying the mitophagy process in both Caenorhabditis elegans and a liver cancer cell line. Cancer Research Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography Dimitri Gaber1, Michal Aharoni-Simon1, Ortal Zaks1, Keren Ben-Yaakov1, Ziv Rotfogel1,2, Hana Leiba1,2, Avital Eisenberg-Lerner*1, Arie L. Marcovich*1,2 1Ophthalmology Research Laboratory, Kaplan Medical Center, 2Department of Ophthalmology, Kaplan Medical Center, Faculty of Medicine, The Hebrew University of Jerusalem The present protocol describes the implantation and evaluation of melanoma in the murine choroid utilizing optical coherence tomography. Neuroscience Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains Ron Refaeli1, Inbal Goshen1 1Edmond and Lily Safra Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem Combining viral vector transduction and brain clearing using the CLARITY method allows the investigation of a large number of neurons and astrocytes simultaneously. Biochemistry Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time Sofia Zaer1, Eitan Lerner1,2 1Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Faculty of Mathematics & Science, The Edmond J. Safra Campus, The Hebrew University of Jerusalem, 2The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem Time-resolved single-molecule protein-induced fluorescence enhancement is a useful fluorescence spectroscopic proximity sensor sensitive to local structural changes in proteins. Here we show it can be used to uncover stable local conformations in α-Synuclein, which is otherwise known as globularly unstructured and unstable when measured using the longer range FRET ruler. Environment A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions Ahan Dalal1, Itamar Shenhar1, Ronny Bourstein1, Amir Mayo1, Yael Grunwald1, Nir Averbuch1, Ziv Attia1, Rony Wallach1, Menachem Moshelion1 1Faculty of Agriculture, Food and Environment, The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem This high-throughput, telemetric, whole-plant water relations gravimetric phenotyping method enables direct and simultaneous real-time measurements, as well as the analysis of multiple yield-related physiological traits involved in dynamic plant–environment interactions.
