Vimta Labs Ltd. 1 article published in JoVE Immunology and Infection Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles Praveen Kumar Balne1,2, Veonice Bijin AU3, Louis Tong2, Arkasubhra Ghosh4, Mukesh Agrawal5, John Connolly3, Rupesh Agrawal1 1National Healthcare Group Eye Institute, Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), 3Institute of Molecular and Cell Biology, A*STAR, 4GROW Research Laboratory, Narayana Nethralaya Foundation, 5Vimta Labs Limited Analysis of tear film cytokines helps in studying various ocular diseases. Bead based multiplex assays are simple and sensitive and enable the testing of multiple targets in samples with small volumes. Here we describe a protocol for tear film cytokine profiling a using bead based multiplex assay.