National Cancer Center Research Institute 3 articles published in JoVE Biology Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1 Yuko Miyagawa1, Kazunori Nagasaka1, Kaoru Yamawaki2,3, Yutaro Mori2,3, Tatsuya Ishiguro2, Kei Hashimoto1, Ryoko Koike1, Siho Fukui1, Takeru Sugihara1, Takayuki Ichinose1, Haruko Hiraike1, Koichiro Kido1, Koji Okamoto3, Takayuki Enomoto2, Takuya Ayabe1 1Department of Obstetrics and Gynecology, Teikyo University School of Medicine, 2Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, 3Devision of Cancer Differentiation, National Cancer Center Research Institute Ovarian cancer stem cells (OCSC) are responsible for cancer initiation, recurrence, therapeutic resistance, and metastasis. The OCSC vascular niche is considered to promote self-renewal of OCSCs, leading to chemoresistance. This protocol provides the basis for establishing a reproducible OCSC vascular niche model in vitro. Cancer Research Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry Ami Maruyama1,2, Kenjiro Kami3, Kazunori Sasaki3, Hajime Sato3, Yuzo Sato1,2, Katsuya Tsuchihara4, Hideki Makinoshima1,2,4 1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory, National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center The purpose of this article is to describe a protocol for extraction of aqueous metabolites from cultured adherent cells for metabolomic analysis, particularly, capillary electrophoresis-mass spectrometry. Genetics Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein Yoshiko Maida*1, Mami Yasukawa*1, Marco Ghilotti1, Yoshinari Ando1, Kenkichi Masutomi1 1Division of Cancer Stem Cell, National Cancer Center Research Institute Human telomerase reverse transcriptase (TERT) synthesizes not only telomeric DNA but also double-stranded RNA through RNA-dependent RNA polymerase activity. Here, we describe a newly established assay to detect RNA-dependent RNA polymerase activity of endogenous TERT.