Teikyo University 3 articles published in JoVE Biology Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1 Yuko Miyagawa1, Kazunori Nagasaka1, Kaoru Yamawaki2,3, Yutaro Mori2,3, Tatsuya Ishiguro2, Kei Hashimoto1, Ryoko Koike1, Siho Fukui1, Takeru Sugihara1, Takayuki Ichinose1, Haruko Hiraike1, Koichiro Kido1, Koji Okamoto3, Takayuki Enomoto2, Takuya Ayabe1 1Department of Obstetrics and Gynecology, Teikyo University School of Medicine, 2Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, 3Devision of Cancer Differentiation, National Cancer Center Research Institute Ovarian cancer stem cells (OCSC) are responsible for cancer initiation, recurrence, therapeutic resistance, and metastasis. The OCSC vascular niche is considered to promote self-renewal of OCSCs, leading to chemoresistance. This protocol provides the basis for establishing a reproducible OCSC vascular niche model in vitro. Behavior Observing the Transformation of Bodily Self-consciousness in the Squeeze-machine Experiment Mai Minoura1, Iori Tani2, Takahiro Ishii3, Yukio- Pegio Gunji1 1Department of Intermedia Art and Science, Waseda University, 2Research Center for Kansei Value Creation, School of Science and Technology, Kwansei Gakuin University, 3Department of Occupational Therapy, Teikyo University of Science The squeeze machine, a V-shaped device that provides pressure along the whole body is used in therapy especially for people with autism. Here, we focus on its possible use in experiments to capture the typical transformations of bodily self-consciousness with peri-personal space measurement. Biochemistry Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species Kotaro Hama1, Yuko Fujiwara1, Kazuaki Yokoyama1 1Faculty of Pharmaceutical Sciences, Teikyo University Here, we present a protocol to quantify and qualify each sphingomyelin species using multiple reaction monitoring and MS/MS/MS mode, respectively.