Kissei Comtech Co., Ltd 1 article published in JoVE Neuroscience Polygraphic Recording Procedure for Measuring Sleep in Mice Yo Oishi1, Yohko Takata1, Yujiro Taguchi2, Sayaka Kohtoh2, Yoshihiro Urade1, Michael Lazarus1 1International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 2Public Sector/Medical Solutions, Kissei Comtech Co., Ltd The recording of electroencephalogram (EEG) and electromyogram (EMG) in freely behaving mice is a critical step to correlate behavior and physiology with sleep and wakefulness. The experimental protocol described herein provides a cable-based system for acquiring EEG and EMG recordings in mice.