Kansai Medical University Graduate School of Medicine 1 article published in JoVE Developmental Biology An Automated Culture System for Maintaining and Differentiating Human-Induced Pluripotent Stem Cells Kazunori Bando1, Hiromi Yamashita1, Fumiyuki Hattori1 1Innovative Regenerative Medicine, Kansai Medical University Graduate School of Medicine Here, we present a protocol for an automated cell culture system. This automated culture system reduces labor and benefits the users, including researchers unfamiliar with handling induced pluripotent stem (iPS) cells, from the maintenance of iPS cells to differentiation into various types of cells.