Korea Advanced Institute of Science and Technology 2 articles published in JoVE Medicine Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window Inwon Park1, Pilhan Kim2,3 1Department of Emergency Medicine, Seoul National University Bundang Hospital, 2Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, 3KI for Health Science and Technology, Korea Advanced Institute of Science and Technology In vivo high-resolution imaging of the pancreas was facilitated with the pancreatic intravital imaging window. Genetics Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast Hogyu David Seo1, Daeyoup Lee1 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology This article describes a detailed methodology for random mutagenesis of a target gene in fission yeast. As an example, we target rpt4+, which encodes a subunit of the 19S proteasome, and screen for mutations that destabilize heterochromatin.