Ewha Womans University View Institution's Website 5 articles published in JoVE Neuroscience Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording Youjin Lee*1,2, Daeho Ryu*3, Saeyeong Jeon3, Yena Lee1, Yoon Kyung Cho1, Chang-Hyeon Ji1,2, Yong-Kweon Kim3,4, Sang Beom Jun1,2,5 1Department of Electronic and Electrical Engineering, Ewha Womans University, 2Graduate Program in Smart Factory, Ewha Womans University, 3Department of Electrical and Computer Engineering, Seoul National University, 4Graduate School of Engineering Practice, Seoul National University, 5Department of Brain and Cognitive Sciences, Ewha Womans University Here, we present the fabrication method of an optrode system with optical fibers for light delivery and an electrode array for neural recording. In vivo experiments with transgenic mice expressing channelrhodopsin-2 show the feasibility of the system for simultaneous optogenetic stimulation and electrophysiological recording. Behavior Transcranial Direct Current Stimulation for Online Gamers Sang Hoon Lee*1, Jooyeon Jamie Im*2, Jin Kyoung Oh2, Eun Kyoung Choi2, Sujung Yoon3, Marom Bikson4, In-Uk Song5, Hyeonseok Jeong2, Yong-An Chung2 1Department of Radiology, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 2Department of Radiology, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 3Department of Brain and Cognitive Sciences, Ewha Womans University, 4Department of Biomedical Engineering, The City College of New York, 5Department of Neurology, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea We present a protocol and a feasibility study for applying transcranial direct current stimulation (tDCS) and neuroimaging assessment in online gamers. Behavior Applying an eMASS Customization Program as a Research Tool to Evaluate Consumer Benefits Minjung Park*1, Jungmin Yoo*2 1Department of Fashion Industry, Ewha Womans University, 2 Presented here is a protocol to examine consumer responses toward mass customization in the context of online retailing. The protocol details the online survey procedure and how to analyze data using structural equation modeling and group differences using latent mean analyses. Neuroscience Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals Sang Beom Jun1,2, Verginia Cuzon Carlson1, Stephen Ikeda3, David Lovinger1 1Section on Synaptic Pharmacology/Laboratory for Integrative Neuroscience, National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism, 2Department of Electronics Engineering, Ewha Womans University, 3Section on Transmitter Signaling/Laboratory of Molecular Physiology, National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism This report demonstrates a technique for mechanical isolation of individual viable neurons retaining attached presynaptic boutons. Vibrodissociated neurons have the advantages of rapid production, excellent pharmacological control and improved space-clamp without influence from neighboring cells. This method can be used for imaging of synaptic elements and patch-clamp recording. Medicine A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium Douglas Nam1, Chih-Wen Ni2, Amir Rezvan1, Jin Suo2, Klaudia Budzyn1, Alexander Llanos1, David G. Harrison1, Don P. Giddens2, Hanjoong Jo1,2,3 1Department of Medicine, Division of Cardiology, Emory University, 2Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, 3Department of Bioinspired Science, Ewha Womans University This describes a partial carotid ligation surgery, which causes disturbed flow conditions and subsequent atherosclerosis development (in two weeks) with intraplaque neo-vascularization (in four weeks) in the mouse common carotid artery. We also describe a novel method of RNA isolation from the carotid intima, providing high purity endothelial RNA.