Shanxi Academy of Agricultural Sciences 1 article published in JoVE Biology Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants Xiaohong Zhu1, Aaron Taylor2, Shenyu Zhang1, Dayong Zhang1,3, Ying Feng1,4, Gaimei Liang1,5, Jian-Kang Zhu1,6 1Department of Horticulture and Landscape Architecture, Purdue University, 2Bindley Bioscience Center, Purdue University, 3Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, 4College of Environmental & Resource Science, Zhejiang University, 5Dryland Agriculture Research Centre, Shanxi Academy of Agricultural Sciences, 6Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences Ca2+ signaling regulates diverse biological processes in plants. Here we present approaches for monitoring abiotic stress induced spatial and temporal Ca2+ signals in Arabidopsis cells and tissues using the genetically encoded Ca2+ indicators Aequorin or Case12.