Tokyo Women's Medical University School of Medicine 2 articles published in JoVE Cancer Research Lung Tumor Cell Recruitment Assay Takeshi Tomita1,2, Katsuaki Ieguchi1, Atsuko Deguchi1, Morichika Takita1, Fujiko Tsukahara1, Sachie Hiratsuka1,2, Yoshiro Maru1 1 This manuscript describes a method to quantify tumor cell accumulation in the lungs in an animal model of tumor metastasis. Medicine Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model Yuka Kato1, Takanori Iwata2, Kaoru Washio2, Toshiyuki Yoshida2, Hozue Kuroda2, Shunichi Morikawa3, Mariko Hamada1, Kazuki Ikura1, Nobuyuki Kaibuchi2, Masayuki Yamato2, Teruo Okano2, Yasuko Uchigata1 1 Adipose-derived stem cells (ASCs) are easily isolated and harvested from the fat of normal rats. ASC sheets can be created using cell-sheet engineering and can be transplanted into Zucker diabetic fatty rats exhibiting full-thickness skin defects with exposed bone and then covered with a bilayer of artificial skin.