Ludwig Boltzmann Institute Applied Diagnostics 1 article published in JoVE Chemistry Technical Aspect of the Automated Synthesis and Real-Time Kinetic Evaluation of [11C]SNAP-7941 Chrysoula Vraka1, Verena Pichler1, Sarah Pfaff1, Theresa Balber1,2, Marcus Hacker1, Markus Mitterhauser1,3, Wolfgang Wadsak1,4, Cecile Philippe1 1Department of Biomedical Imaging and Image-guided Therapy, Division of Nuclear Medicine, Medical University of Vienna, 2Department for Pharmaceutical Technology and Biopharmaceutics, Faculty of Life Sciences, University of Vienna, 3Ludwig Boltzmann Institute Applied Diagnostics, 4CBmed GmbH - Center for Biomarker Research in Medicine Here, we represent a protocol for the fully automated radiolabelling of [11C]SNAP-7941 and the analysis of the real-time kinetics of this PET-tracer on P-gp expressing and non-expressing cells.