Ectica Technologies AG 1 article published in JoVE Bioengineering Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate Lisa A. Krattiger1, Maria Mitsi2, Benjamin R. Simona2, Martin Ehrbar1 1Department of Obstetrics, University Hospital Zurich, University of Zurich, 2Ectica Technologies AG An in vitro model of the bone marrow vascular niche is established by seeding mesenchymal and endothelial cells onto pre-cast 3D PEG hydrogels. The endothelial networks, ECM components, and ALP activity of the niches vary depending on the growth factor used. The platform can be used for advanced cancer models.