Signalling Research Centres BIOSS and CIBSS 1 article published in JoVE Biochemistry High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis Catrin Swantje Müller1, Wolfgang Bildl1, Norbert Klugbauer2, Alexander Haupt1, Bernd Fakler1,3, Uwe Schulte1,3,4 1Institute of Physiology, Faculty of Medicine, University of Freiburg, Freiburg, Germany, 2Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Freiburg, Freiburg, Germany, 3Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, 4Logopharm GmbH, Germany A versatile cryoslicing BN-MS protocol using a microtome is presented for high-resolution complexome profiling.