University of Valencia 5 articles published in JoVE Environment Data Collection on Marine Litter Ingestion in Sea Turtles and Thresholds for Good Environmental Status Marco Matiddi1, Giuseppe A. deLucia2, Cecilia Silvestri1, Gaëlle Darmon3, Jesús Tomás4, Christopher K. Pham5, Andrea Camedda2, Frederic Vandeperre5,6, Françoise Claro7, Yakup Kaska8, Helen Kaberi9, Ohiana Revuelta4, Raffaella Piermarini1, Roberto Daffina1, Marco Pisapia1, Daniela Genta1, Doğan Sözbilen8, Mohamed N. Bradai10, Yasmina Rodríguez5, Delphine Gambaiani3, Catherine Tsangaris9, Olfa Chaieb10, Judicaëlle Moussier7, Ana L. Loza11, Claude Miaud3, INDICIT consortium, 1Italian National Institute for Environmental Protection and Research (ISPRA), 2Institute for Coastal Marine Environment-National Research Council (IAMC-CNR), 3EPHE, PSL Research University, UMR 5175 CE3FE, CNRS, UM, Univ P. Valery, SupAgro, IRD, INRA, Biogéographie et Écologie des Vertébrés, 4Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, 5Departamento de Oceanografia e Pescas, Instituto do Mar/Okeanos, Universidade dos Açores, 6MARE - Marine and Environmental Sciences Centre, Universidade dos Açores, 7 The protocol focuses on the collection of sea turtle samples, describing all the steps from the animal recovery and necropsy to the classification and quantification of ingested marine litter. Moreover, the representative results show how to use the collected data to elaborate the possible thresholds for Good Environmental Status. Biology Fish Sperm Assessment Using Software and Cooling Devices Carina Caldeira1, Carles Soler1,2 1PROiSER R+D S.L., 2Department of Cellular Biology, Functional Biology and Physical Anthropology, University of Valencia The present protocol describes a procedure of fish sperm assessment using computer-assisted sperm analysis and cooling devices. The software gives a rapid, accurate and quantitative analysis of fish sperm quality based on spermatozoa motility, which can be a useful tool in aquaculture to improve reproduction success. Behavior Reinstatement of Drug-seeking in Mice Using the Conditioned Place Preference Paradigm M. Carmen Blanco-Gandía1, María A. Aguilar1, José Miñarro1, Marta Rodríguez-Arias1 1Unidad de Investigación Psicobiología de las Drogodependencias, Departamento de Psicobiología, Facultad de Psicología, Universitat de València This protocol describes the Conditioned Place Preference (CPP) as a model of relapse. This procedure permits the measurement of relapse in laboratory animals, considering the impact of drug-associated environmental cues as craving and relapse in abstaining addicts is currently the focus of drug-abuse treatment programs. Medicine An Experimental Model of Diet-Induced Metabolic Syndrome in Rabbit: Methodological Considerations, Development, and Assessment Óscar Julián Arias-Mutis1,2,3, Patricia Genovés1,2,3, Conrado J. Calvo1,2,4, Ana Díaz5, Germán Parra2,3, Luis Such-Miquel6, Luis Such2, Antonio Alberola2, Francisco Javier Chorro1,3, Manuel Zarzoso6 1CIBERCV, Instituto de Salud Carlos III, 2Department of Physiology, Universitat de València, 3INCLIVA, 4Department of Electronic Engineering, Universidad Politécnica de Valencia, 5UCIM, Universitat de València, 6Department of Physiotherapy, Universitat de València We describe methods to develop an experimental model of diet-induced metabolic syndrome (MetS) in rabbits using a high-fat, high-sucrose diet. Animals developed central obesity, mild hypertension, pre-diabetes, and dyslipidemia, thus reproducing the main components of human MetS. This chronic model will allow acquisition of knowledge underlying mechanisms of disease progression. Biology Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles Estela Selma-Soriano1,2,3, Rubén Artero1,2,3, Beatriz Llamusi1,2,3 1Department of Genetics and Interdisciplinary Research Structure for Biotechnology and Biomedicine (BIOTECMED), University of Valencia, 2Incliva Health Research Institute, 3Joint unit CIPF-Incliva We report a method to quantify muscle area, which is an indirect method to determine muscle mass in Drosophila adults. We demonstrate the application of our methodology by analyzing the indirect flight muscles in a Drosophila model of Myotonic Dystrophy disease.