Groupe Hospitalier Paris Saint Joseph 1 article published in JoVE Medicine Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography Simon Dang Van1,2,3, Dorothée Brunet1,2,3, Ali Akamkam1,2,3, Benoit Decante1,2, Julien Guihaire1,2,3 1Laboratory of Preclinical Research, Department of Research and Innovation, Groupe Hospitalier Paris Saint Joseph, 2Inserm UMR S 999, Pulmonary Hypertension: Pathophysiology and Novel Therapies, Marie Lannelongue Hospital, Groupe Hospitalier Paris Saint Joseph, 3Department of Cardiac Surgery, Marie Lannelongue Hospital A reliable noninvasive approach for functional assessment of the donor heart during normothermic ex situ heart perfusion (NESP) is lacking. We herein describe a protocol for ex situ assessment of myocardial performance using the epicardial echocardiography and conductance catheter method.