Keele University 5 articles published in JoVE Medicine Use of a Percutaneous Ventricular Assist Device/Left Atrium to Femoral Artery Bypass System for Cardiogenic Shock Swethika Sundaravel1, Poonam Velagapudi1, Mamas Mamas2, Sandeep Nathan3, Alexander Truesdell4 1University of Nebraska Medical Center, 2Keele Cardiovascular Research Group, Keele University, 3University of Chicago Medicine, 4Virginia Heart/Inova Heart and Vascular Institute The following article describes the stepwise procedure for placement of a device (e.g., Tandemheart) in cardiogenic shock (CS) that is a percutaneous left ventricular assist device (pLVAD) and a left atrial to femoral artery bypass (LAFAB) system that bypasses and supports the left ventricle (LV) in CS. Biology Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage Yiwei Zhao1, Gene Chi Wai Man1,2, Loucia Kit Ying Chan1, Xi Guo1, Yingyu Liu3, Tao Zhang1, Joseph Kwong1,4, Chi Chiu Wang1,5, Xiaoyan Chen3, Tin Chiu Li1 1Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, 2Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, 3Department of Obstetrics and Gynaecology, Shenzhen Baoan Women’s and Children’s Hospital, Shenzhen University, 4School of Medicine, Faculty of Medicine and Health Sciences, Keele University, 5Li Ka Shing Institute of Health Sciences; School of Biomedical Sciences, The Chinese University of Hong Kong Despite the advancements in multiplex immunohistochemistry and multispectral imaging, characterizing the density and clustering of major immune cells simultaneously in the endometrium remains a challenge. This paper describes a detailed multiplex staining protocol and imaging for the simultaneous localization of four immune cell types in the endometrium. Medicine Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing Solja Kalha1, Alison Kuony1, Frederic Michon1,2 1Helsinki Institute of Life Science, Institute of Biotechnology, University of Helsinki, 2School of Medicine and Institute for Science and Technology in Medicine, Keele University This protocol describes a method to inflict an abrasion to the ocular surface of the mouse, and to follow the wound healing process thereafter. The protocol takes advantage of an ocular burr to partially remove the surface epithelium of the eye in anaesthetized mice. Biochemistry Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline Martha E. Brennich1, Adam R. Round2,3, Stephanie Hutin4 1Structural Biology Group, European Synchrotron Radiation Facility, 2European Molecular Biology Laboratory, 3School of Chemical and Physical Sciences, Keele University, 4Groupe de Microscopie Electronique et Méthodes, Institut de Biologie Structurale The determination of the solution structure of a protein by small angle X-ray scattering (SAXS) requires monodisperse samples. Here, we present two possibilities to ensure minimal delays between sample preparation and data acquisition: online size-exclusion chromatography (SEC) and online ion-exchange chromatography (IEC). Neuroscience Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types Vishal Menon1, Ria Thomas1,2, Arun R. Ghale1,3, Christina Reinhard1, Jan Pruszak1,4 1Emmy Noether-Group for Stem Cell Biology, Department of Molecular Embryology, Institute of Anatomy and Cell Biology, University of Freiburg, 2Spemann Graduate School of Biology and Medicine and Faculty of Biology, University of Freiburg, 3School of Life Sciences, Keele University, 4Center for Biological Signaling Studies (BIOSS), University of Freiburg We provide a detailed description of a protocol for flow cytometric analysis of surface antigens and/or intracellular antigens in neural cell types. Critical aspects of experimental planning, step-by-step methodological procedures, and fundamental principles of flow cytometry are explained in order to enable neurobiologists to exploit this powerful technology.