Shahed University 1 article published in JoVE Neuroscience Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes Maryam Bagheri1, Arjang Rezakhani1, Mehrdad Roghani2, Mohammad T. Joghataei3,4, Simin Mohseni1 1Department of Clinical and Experimental Medicine, Linköping University, 2Neurophysiology Research Center, Shahed University, 3Cellular and Molecular Research Center, Iran University of Medical Sciences, 4School of Advanced Technologies in Medicine, Iran University of Medical Sciences Astrocytes in the CNS change their functional and structural properties in response to harmful stimuli. This report presents a protocol for assessment of three-dimensional astrocyte morphology in diseased conditions or after therapeutic interventions.