National University Hospital 1 article published in JoVE Medicine Measuring Retinal Vessel Diameter from Mouse Fluorescent Angiography Images Andrea García-Llorca1, Hallur Reynisson1, Thor Eysteinsson1,2 1Department of Physiology, Biomedical Center, Faculty of Medicine, University of Iceland, 2Department of Ophthalmology, National University Hospital Mouse retinal vasculature is particularly interesting in understanding the mechanisms of vascular pattern formation. This protocol automatically measures the diameter of mouse retinal vessels from fluorescent angiography fundus images at a fixed distance from the optic disk.