Riga Technical university 1 article published in JoVE Bioengineering Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device Miks Priedols*1, Gunita Paidere*2, Pauls Kaukis*1, Cristina Bajo-Santos1, Arnita Spule3,4, Antons Miscenko1, Gatis Mozolevskis2,3, Roberts Rimsa2,3, Arturs Abols1,3 1Latvian Biomedical Research and Study Centre, 2Institute of Solid-State Physics, University of Latvia, 3Cellbox labs LTD, Atari, 4Faculty of Materials Science and Applied Chemistry, Institute of General Chemical Engineering, Riga Technical university Extracellular vesicles hold immense promise for biomedical applications, but current isolation methods are time-consuming and impractical for clinical use. In this study, we present a microfluidic device that enables the direct isolation of extracellular vesicles from large volumes of biofluids in a continuous manner with minimal steps.