Wroclaw University of Science and Technology 3 articles published in JoVE Chemistry Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization Maciej Lipok1, Patryk Obstarczyk1, Joanna Olesiak-Bańska1 1Institute of Advanced Materials, Wrocław University of Science and Technology This paper describes how polarization-sensitive two-photon microscopy could be applied to characterize the local organization within label-free amyloid superstructures-spherulites. It also describes how to prepare and measure the sample, assemble the required setup, and analyze the data to obtain information about the local organization of amyloid fibrils. Engineering Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction Andrzej Żak1, Olga Kaczmarczyk2 1Electron Microscopy Laboratory, Faculty of Mechanical Engineering, Wroclaw University of Science and Technology, 2Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Science and Technology The present protocol describes transmission electron microscope (TEM) modifications with a light illumination system, the fabrication of liquid cells, and in situ TEM observations of light-induced interactions between bacterial cells and a photosensitizer. The sample preparation methods, electron beam damage, and imaging are also discussed. Bioengineering Conducting Multiple Imaging Modes with One Fluorescence Microscope Seongjin Park1, Jiacheng Zhang2, Matthew A. Reyer2, Joanna Zareba1,3, Andrew A. Troy4, Jingyi Fei1,2 1Department of Biochemistry and Molecular Biology, University of Chicago, 2The Institute for Biophysical Dynamics, University of Chicago, 3Faculty of Chemistry, Wrocław University of Science and Technology, 4Nikon Instruments Inc. Here we present a practical guide of building an integrated microscopy system, which merges conventional epi-fluorescent imaging, single-molecule detection-based super-resolution imaging, and multi-color single-molecule detection, including single-molecule fluorescence resonance energy transfer imaging, into one set-up in a cost-efficient way.