Universidade do Algarve, Campus de Gambelas 2 articles published in JoVE Environment Extracellular Multi-Unit Recording from the Olfactory Nerve of Teleosts Peter Hubbard1, Zélia Velez1,2 1Centro de Ciências do Mar, 2Universidade do Algarve Extracellular multi-unit recording from the olfactory nerve is a sensitive, robust and reproducible method for assessing olfactory sensitivity in marine fish. It records primary sensory input and is independent of external salinity. Biology A Blood-Free Diet to Rear Anopheline Mosquitoes Joana Marques1, João C. R. Cardoso2, Rute C. Félix2, Deborah M. Power2, Henrique Silveira1 1Global Health and Tropical Medicine, GHTM, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, IHMT-NOVA, 2Comparative Endocrinology and Integrative Biology, Centre of Marine Sciences, Universidade do Algarve, Campus de Gambelas A protocol is presented for formulation of a blood-free artificial diet to feed Anopheles mosquitoes in captivity. This diet has a similar performance to vertebrate blood and triggers oogenesis and egg maturation and produces viable adult progeny.