Bavarian Health and Food Safety Authority 1 article published in JoVE Biology Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration Florian Christof Bonnaire1,2, Martina Feierabend3, Julius Michael Wolfgart1,4, Wolfram Breuer5, Christian Walter2, Ulf Krister Hofmann1,2, Marina Danalache1 1Laboratory of Cell Biology, Department of Orthopedic Surgery, University Hospital of Tübingen, 2Department of Orthopedic Surgery, University Hospital of Tübingen, 3Institute for Bioinformatics and Medical Informatics, Faculty of science of the University of Tübingen, 4Medical faculty of the University of Tübingen, 5Bavarian Health and Food Safety Authority We present a method to investigate spatial chondrocyte organization in the anulus fibrosus of the intervertebral disc using an optical sectioning method.