Volda University College 1 article published in JoVE Neuroscience Neuropharmacological Manipulation of Restrained and Free-flying Honey Bees, Apis mellifera Eirik Søvik*1,2, Jenny A. Plath*3,4, Jean-Marc Devaud5, Andrew B. Barron3 1Department of Science and Mathematics, Volda University College, 2Department of Biology, Washington University in St. Louis, 3Department of Biological Sciences, Macquarie University, 4Department of Biology, University of Konstanz, 5Research Center on Animal Cognition, CNRS, Universite de Toulouse This manuscript describes several protocols for administering pharmacological agents to honey bees, including simple noninvasive methods for free-flying bees, as well as more invasive variants that allow precise localized treatment of restrained bees.