BioTime, Inc 1 article published in JoVE Developmental Biology Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina Ratnesh K. Singh1, Pablo E. Diaz1, François Binette1, Igor O. Nasonkin1 1BioTime, Inc The objective of this report is to describe the protocol for robust immunohistochemical detection of epigenetic markers, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in developing and postmitotic mouse retina.