Colorado State University College of Veterinary Medicine and Biomedical Sciences 1 article published in JoVE Neuroscience Subcellular Imaging of Neuronal Calcium Handling In Vivo Rachel Doser*1, Kaz M. Knight*1,2, Ennis Deihl1, Frederic Hoerndli1 1Department of Biomedical Sciences, Colorado State University College of Veterinary Medicine and Biomedical Sciences, 2Cellular and Molecular Biology Graduate Program, Colorado State University College of Veterinary Medicine and Biomedical Sciences The current methods describe a non-ratiometric approach for high-resolution, sub-compartmental calcium imaging in vivo in Caenorhabditis elegans using readily available genetically encoded calcium indicators.