Bio-Rad Laboratories, Inc. View Institution's Website 5 articles published in JoVE Biology V3 Stain-free Workflow for a Practical, Convenient, and Reliable Total Protein Loading Control in Western Blotting Anton Posch1, Jonathan Kohn1, Kenneth Oh1, Matt Hammond1, Ning Liu1 1Bio-Rad Laboratories V3 workflow is a western blot procedure using stain-free gels. The stain-free technology allows researchers to visualize protein separation quality, to verify the transfer efficiency, and most importantly, to validate the change in the protein of interest using total protein quantification as a reliable loading control. Application Notes Simultaneous, Rapid, and Highly Efficient Protein Transfer Using the Trans-Blot Turbo Transfer System - ADVERTISEMENT Nikolas H. Chmiel1, Paul Z. Liu1, Xiaoyi Xu1, Lawreen L. Asuncion1, Christopher M. Belisle1 1Bio-Rad Laboratories The Trans-Blot Turbo system reduces protein transfer protocols from gels to as little as 3 minutes, while maintaining high efficiency transfers and high throughput. The system enables protein transfer of 2 mini gels in 3 minutes and up to 4 mini gels in as little as 7 minutes. Biology Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells Adam M. McCoy1, Claudia Litterst1, Michelle L. Collins1, Luis A. Ugozzoli1 1Gene Expression Division, Bio-Rad Laboratories This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling. Biology Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency Adam M. McCoy1, Michelle L. Collins1, Luis A. Ugozzoli1 1Gene Expression Division, Bio-Rad Laboratories, Inc. This procedure shows how to use the Gene Pulser MXcell electroporation system to rapidly and easily identify the best electroporation conditions for mouse embryonic fibroblasts (MEFs) or other primary cells. Considerations for troubleshooting are also discussed in the associated video. Biology The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation Kelly Kroeger1, Michelle Collins1, Luis Ugozzoli1 1Gene Expression Division, Bio-Rad Laboratories, Inc This procedure describes how to establish primary hematopoietic cell cultures from murine bone marrow and is followed by transfection using the Gene Pulser MXCell electroporation system.