Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill 1 article published in JoVE Medicine Isolation and Culture Expansion of Tumor-specific Endothelial Cells Lin Xiao1, James V. McCann1, Andrew C. Dudley1,2,3 1Department of Cell Biology & Physiology, University of North Carolina at Chapel Hill, 2Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 3McAllister Heart Institute, University of North Carolina at Chapel Hill We report a reliable method to isolate and culture primary tumor-specific endothelial cells from genetically engineered mouse models.